CyFlow™ CD11b Biotin

Antigen:	CD11b
Alternative Name:	Mac-1
Clone:	ICRF44
Application:	Flow cytometry
Format/Fluorochrome:	Biotin
Target Species:	Human, Non-Human Primates
Field of Interest:	Immunophenotyping
Species of Origin:	Mouse
Regulatory Status:	RUO
Clonality:	monoclonal
Isotype:	IgG1
Order number:	CV909688

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA IV—WS Code M047 Concentration Unit mg/mL Concentration 1... more

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CyFlow™ CD11b Biotin

HLDA Workshop	HLDA IV—WS Code M047
Concentration Unit	mg/mL
Concentration	1
Quantity	0.1 mg
Volume	0.1
Immunogen	Rheumatoid synovial cells and human monocytes
Background Information	CD11b (integrin αM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with CD18 (integrin β2 subunit); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Usage	The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
Storage Buffer	The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
Storage	Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
Stability	Do not use after expiration date stamped on vial label.

Specific References

| Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP‑1alpha) and MIP‑1beta production in primary human monocytes through a pathway dependent on nuclear factor‑kappaB. Blood. 2001·May·15; 97(10):2932‑40. <·PMID:·11342414·> | Sánchez-Torres C, García-Romo GS, Cornejo-Cortés MA, Rivas-Carvalho A, Sánchez-Schmitz G: CD16+ and CD16‑ human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int·Immunol. 2001·Dec; 13(12):1571‑81. <·PMID:·11717198·> | Hua J, Sakamoto K, Nagaoka I: Inhibitory actions of glucosamine, a therapeutic agent for osteoarthritis, on the functions of neutrophils. J·Leukoc·Biol. 2002·Apr; 71(4):632‑40. <·PMID:·11927650·> | Masten BJ, Olson GK, Tarleton CA, Rund C, Schuyler M, Mehran R, Archibeque T, Lipscomb MF: Characterization of myeloid and plasmacytoid dendritic cells in human lung. J·Immunol. 2006·Dec·1; 177(11):7784‑93. <·PMID:·17114449·> | Tieu BC, Lee C, Sun H, Lejeune W, Recinos A 3rd, Ju X, Spratt H, Guo DC, Milewicz D, Tilton RG, Brasier AR: An adventitial IL‑6/MCP1 amplification loop accelerates macrophage‑mediated vascular inflammation leading to aortic dissection in mice. J·Clin·Invest. 2009·Dec; 119(12):3637‑51. <·PMID:·19920349·>

Welcome toSysmex Flow Cytometry Ireland!

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Sysmex Flow Cytometry Ireland!