CyFlow™ CD3 Pacific Blue™
| Antigen: | CD3 |
| Alternative Name: | Leu4, T3 |
| Clone: | UCHT1 |
| Application: | Flow cytometry |
| Format/Fluorochrome: | Pacific Blue™ |
| Laser: | Violet |
| Target Species: | Human, Non-Human Primates |
| Field of Interest: | Immunophenotyping |
| Species of Origin: | Mouse |
| Regulatory Status: | RUO |
| Clonality: | monoclonal |
| Emission Maximum: | 455 nm |
| Excitation Maximum: | 400 to 410 nm |
| Isotype: | IgG1 |
| Order number: | CN914378 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
| HLDA Workshop | HLDA I—WS Code T 3 HLDA III—WS Code T 126 HLDA III—WS Code T 471 HLDA VI—WS Code T 6T-CD3.1 |
| Quantity | 100 tests |
| Volume | 0.4 |
| Immunogen | Human thymocytes followed by Sezary T cells |
| Background Information | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Garson JA, Beverley PC, Coakham HB, Harper EI: Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982·Jul·22; 298(5872):375‑7. <·PMID:·6178042·> | McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·> | Fisch P, Malkovsky M, Braakman E, Sturm E, Bolhuis RL, Prieve A, Sosman JA, Lam VA, Sondel PM: Gamma/delta T cell clones and natural killer cell clones mediate distinctpatterns of non‑major histocompatibility complex‑restricted cytolysis. J·Exp·Med. 1990·May·1; 171(5):1567‑79. <·PMID:·2185329·> | Barclay, Brown, et al.: The Leukocyte Antigen FactsBook; 2nd edition. Harcourt·Brace·&·Company,·London. 1997; <·No·PMID·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | le Gouvello S, Manceau V, Sobel A: Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin‑dependent kinase II after CD2 triggering of human T lymphocytes. J·Immunol. 1998·Aug·1; 161(3):1113‑22. <·PMID:·9686569·> | Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J·Immunol. 2003·Jun·15; 170(12):5947‑55. <·PMID:·12794121·> | Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J·Biol·Chem. 2004·Jul·9; 279(28):28827‑3. <·PMID:·15084594·> | Arnett KL, Harrison SC, Wiley DC: Crystal structure of a human CD3‑epsilon/delta dimer in complex with a UCHT1 single‑chain antibody fragment. Proc·Natl·Acad·Sci·USA. 2004·Nov·16; 101(46):16268‑73. <·PMID:·15534202·> | Demedts IK, Brusselle GG, Vermaelen KY, Pauwels RA: Identification and characterization of human pulmonary dendritic cells. Am·J·Respir·Cell·Mol·Biol. 2005·Mar; 32(3):177‑84. <·PMID:·15576669·> | Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005·Nov·15; 106(10):3567‑74. <·PMID:·16046525·> | Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006·Feb; 24(2):133‑9. <·PMID:·16473826·> | Alarcón B, Swamy M, van Santen HM, Schamel WW: T‑cell antigen‑receptor stoichiometry: pre‑clustering for sensitivity. EMBO·Rep. 2006·May; 7(5):490‑5. <·PMID:·16670682·> | Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T‑cell deficiency. N·Engl·J·Med. 2006·May·4; 354(18):1913‑21. <·PMID:·16672702·> | Siegers GM, Swamy M, Fernández-Malavé E, Minguet S, Rathmann S, Guardo AC, Pérez-Flores V, Regueiro JR, Alarcón B, Fisch P, Schamel WW: Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J·Exp·Med. 2007·Oct·29; 204(11):2537‑44. <·PMID:·17923503·>