CyFlow™ CD2 APC
| Antigen: | CD2 |
| Alternative Name: | LFA-2 |
| Clone: | LT2 |
| Application: | Flow cytometry |
| Format/Fluorochrome: | APC |
| Laser: | Red |
| Target Species: | Human |
| Field of Interest: | Immunophenotyping |
| Species of Origin: | Mouse |
| Regulatory Status: | RUO |
| Clonality: | monoclonal |
| Emission Maximum: | 660 nm |
| Excitation Maximum: | 650 nm |
| Isotype: | IgG2b |
| Order number: | CG364305 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
| HLDA Workshop | HLDA VI—WS Code T 6T-008 |
| Quantity | 100 tests |
| Volume | 1 |
| Immunogen | Normal human blood lymphocytes |
| Background Information | CD2 (LFA-2) belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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