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CyFlow™ CD25 Low Endotoxin

CyFlow™ CD25 Low Endotoxin
Antigen: CD25
Alternative Name: IL-2R, p55, TAC
Clone: PC61.5
Application: Flow cytometry, Functional assays, Immunohistochemistry (frozen sections), Immunoprecipitation
Format/Fluorochrome: Low Endotoxin
Target Species: Mouse
Field of Interest: Immunophenotyping, MHC
Species of Origin: Rat
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: BF390879

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration 1 mg/ml Quantity 0.1 mg Volume 0.1 ml Immunogen B6.1 CTL cell line... more
CyFlow™ CD25 Low Endotoxin
Concentration1 mg/ml
Quantity0.1 mg
Volume0.1 ml
ImmunogenB6.1 CTL cell line
SpecifityThe rat monoclonal antibody PC61.5 (PC61.5.3) recognizes mouse CD25 antigen, a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
Background InformationCD25 (IL2Rα, Tac) is a ligand-binding α subunit of interleukin 2 receptor (IL2R). Together with β and γ subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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