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CyFlow™ CD18 Low Endotoxin

CyFlow™ CD18 Low Endotoxin
Antigen: CD18
Alternative Name: b2 integrin
Clone: MEM-148
Application: Flow cytometry, Functional assays, Immunoprecipitation, Western blot
Format/Fluorochrome: Low Endotoxin
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: AH284465

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 Immunogen... more
CyFlow™ CD18 Low Endotoxin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1
ImmunogenPeripheral blood mononuclear cells
Background InformationCD18 (integrin β2 subunit) forms heterodimers with four types of CD11 molecule to constitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1, CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Gao JX, Issekutz AC: Mac‑1 (CD11b/CD108) is the predominant beta 2 (CD108) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers. Immunology. 1996·Jul; 88(3):463‑70. <·PMID:·8774366·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Shang XZ, Issekutz AC: Contribution of CD11a/CD18, CD11b/CD18, ICAM‑1 (CD54 and ‑2 (CD102 to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur·J·Immunol. 1998·Jun; 28(6):1970‑9. <·PMID:·9645379·> | Drbal K, Angelisova P, Cerny J, Pavlistova D, Cebecauer M, Novak P, Horejsi V: Human leukocytes contain a large pool of free forms of CD18. Biochem·Biophys·Res·Commun. 2000·Aug·28; 275(2):295‑9. <·PMID:·10964661·> | Drbal K, Angelisova P, Cerny J, Hilgert I, Horejsi V: A novel anti‑CD18 mAb recognizes an activation‑related epitope and induces a high‑affinity conformation in leukocyte integrins. Immunobiology. 2001·May; 203(4):687‑98. <·PMID:·11402502·> | Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001·Sep·1; 98(5):1561‑6. <·PMID:·11520808·> | Kess D, Peters T, Zamek J, Wickenhauser C, Tawadros S, Loser K, Varga G, Grabbe S, Nischt R, Sunderkötter C, Müller W, Krieg T, Scharffetter-Kochanek K: CD4+ T cell‑associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis. J·Immunol. 2003·Dec·1; 171(11):5697‑706. <·PMID:·14634077·> | Solovjov DA, Pluskota E, Plow EF: Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2. J·Biol·Chem. 2005·Jan·14; 280(2):1336‑45. <·PMID:·15485828·> | Peters T, Sindrilaru A, Wang H, Oreshkova T, Renkl AC, Kess D, Scharffetter-Kochanek K: CD18 in monogenic and polygenic inflammatory processes of the skin. J·Investig·Dermatol·Symp·Proc. 2006·Sep; 11(1):42186. <·PMID:·17069006·> | Dassanayake RP, Maheswaran SK, Srikumaran S: Monomeric expression of bovine beta2‑integrin subunits reveals their role in Mannheimia haemolytica leukotoxin‑induced biological effects. Infect·Immun. 2007·Oct; 75(10):5004‑10. <·PMID:·17698568·> | Aubert M, Yoon M, Sloan DD, Spear PG, Jerome KR: The virological synapse facilitates herpes simplex virus entry into T cells. J·Virol. 2009·Jun; 83(12):6171‑83. <·PMID:·19339346·> | Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. eLife. 2015·Dec·9; 4:e10766. <·PMID:·26650353·> | Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund‐Johansen F, Kalina T: High‐resolution antibody array analysis of childhood acute leukemia cells. Mol·Cell·Proteomics. 2016·Apr·1; 15(4):1246‐61. <·PMID:·26785729·>

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