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CyFlow™ CD14 Biotin

CyFlow™ CD14 Biotin
Antigen: CD14
Alternative Name: LPS-R
Clone: MEM-18
Application: Flow cytometry
Format/Fluorochrome: Biotin
Target Species: Human, Non-Human Primates
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: CV242434

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration 1 mg/ml Quantity 0.1 mg Volume 0.1 ml Immunogen A crude mixture of... more
CyFlow™ CD14 Biotin
Concentration1 mg/ml
Quantity0.1 mg
Volume0.1 ml
ImmunogenA crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
SpecifityThe mouse monoclonal antibody MEM-18 recognizes CD14 antigen, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
Background InformationCD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 1·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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