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CyFlow™ CD11c PE-Cy7

CyFlow™ CD11c PE-Cy7
Antigen: CD11c
Alternative Name: p150
Clone: BU15
Application: Flow cytometry
Format/Fluorochrome: PE-Cy7
Laser: Blue, Green, Yellow
Target Species: Human, Non-Human Primates
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 785 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG1
Order number: AL905427

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA III—WS Code M 256 HLDA V—WS Code AS S143 HLDA VI—WS Code AS Ref.6... more
CyFlow™ CD11c PE-Cy7
HLDA WorkshopHLDA III—WS Code M 256 HLDA V—WS Code AS S143 HLDA VI—WS Code AS Ref.6
Quantity100 tests
Volume0.4
ImmunogenDendritic cells of synovial fluid
Background InformationCD11c (αX integrin subunit) forms complex with CD18 (β2 integrin subunit) and is expressed mainly on tissue macrophages and dendritic cells. CD11c binds to complement fragment iC3b, fibrinogen, VCAM-1 and ICAM-2 or e.g. CD90. Like other β2 integrins, CD11c/CD18 plays roles in cell migration and phagocytosis. Moreover, interaction of CD11c/CD18 with plasminogen regulates plasmin activities, and interaction with heparin counteracts binding of iC3b.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Hogg N, Takacs L, Palmer DG, Selvendran Y, Allen C: The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur·J·Immunol. 1986·Mar; 16(3):240‑8. <·PMID:·3456894·> | McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Trowald-Wigh G, Johannisson A, Håkansson L: Canine neutrophil adhesion proteins and Fc‑receptors in healthy dogs and dogs with adhesion protein deficiency, as studied by flow cytometry. Vet·Immunol·Immunopathol. 1993·Oct; 38(3‑4):297‑310. <·PMID:·8291207·> | Van der Vieren M, Le Trong H, Wood CL, Moore PF, St John T, Staunton DE, Gallatin WM: A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM‑3. Immunity. 1995·Dec; 3(6):683‑90. <·PMID:·8777714·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP‑1alpha) and MIP‑1beta production in primary human monocytes through a pathway dependent on nuclear factor‑kappaB. Blood. 2001·May·15; 97(10):2932‑40. <·PMID:·11342414·> | Myou S, Zhu X, Boetticher E, Qin Y, Myo S, Meliton A, Lambertino A, Munoz NM, Hamann KJ, Leff AR: Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2‑integrin caused by ERK‑independent activation of cPLA2. Immunology. 2002·Sep; 107(1):77‑85. <·PMID:·12225365·> | Bullard DC, Hu X, Adams JE, Schoeb TR, Barnum SR: p150/95 (CD11c/CD18 expression is required for the development of experimental autoimmune encephalomyelitis. Am·J·Pathol. 2007·Jun; 170(6):2001‑8. <·PMID:·17525267·> | Sadhu C, Ting HJ, Lipsky B, Hensley K, Garcia-Martinez LF, Simon SI, Staunton DE: CD11c/CD18: novel ligands and a role in delayed‑type hypersensitivity. J·Leukoc·Biol. 2007·Jun; 81(6):1395‑403. <·PMID:·17389580·> | Vorup-Jensen T, Chi L, Gjelstrup LC, Jensen UB, Jewett CA, Xie C, Shimaoka M, Linhardt RJ, Springer TA: Binding between the integrin alphaXbeta2 (CD11c/CD18 and heparin. J·Biol·Chem. 2007·Oct·19; 282(42):30869‑77. <·PMID:·17699512·> | Gang J, Choi J, Lee JH, Nham SU: Identification of critical residues for plasminogen binding by the alphaXI‑domain of the beta2 integrin, alphaXbeta2. Mol·Cells. 2007·Oct·31; 24(2):240‑6. <·PMID:·17978577·> | Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR: CD14+ antigen‑presenting cells in human dermis are less mature than their CD1a+ counterparts. Int·Immunol. 2007·Nov; 19(11):1271‑9. <·PMID:·17804688·> | Sadhu C, Hendrickson L, Dick KO, Potter TG, Staunton DE: Novel Tools for Functional Analysis of CD11c: Activation‑Specific, Activation‑Independent, and Activating Antibodies. J·Immunoassay·Immunochem. 2008; 29(1):42‑57. <·PMID:·18080879·>

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