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CyFlow™ CD8b FITC

CyFlow™ CD8b FITC
Antigen: CD8b
Clone: 341
Application: Flow cytometry
Format/Fluorochrome: FITC
Laser: Blue
Target Species: Rat
Field of Interest: Immunophenotyping, MHC
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgG1
Order number: BP937236

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration Unit mg/mL Concentration 0.5 Quantity 0.1 mg Volume 0.2... more
CyFlow™ CD8b FITC
Concentration Unitmg/mL
Concentration0.5
Quantity0.1 mg
Volume0.2
ImmunogenCD8 positive Wistar rat splenic T cell hybridomas
Background InformationCD8b (CD8 β) subunit of CD8 T cell coreceptor is expressed in CD8 α/β heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 α/α homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α/β but not α/α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur·J·Immunol. 1992·Nov; 22(11):2841‑8. <·PMID:·1358623·> | Kühnlein P, Park JH, Herrmann T, Elbe A, Hünig T: Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J·Immunol. 1994·Aug1; 153(3):979‑86. <·PMID:·8027567·> | Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur·J·Immunol. 1996·Nov; 26(11):2582‑6. <·PMID:·8921942·> | Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J·Immunol. 1997·Feb·15; 158(4):1833‑40. <·PMID:·9029123·> | Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD: Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J·Immunol. 1998·Jun·15; 160(12):6004‑11. <·PMID:·9637515·> | Lin TJ, Hirji N, Nohara O, Stenton GR, Gilchrist M, Befus AD: Mast cells express novel CD8 molecules that selectively modulate mediator secretion. J·Immunol. 1998·Dec·1; 161(11):6265‑72. <·PMID:·9834115·> | Ellerman KE, Like AA: Islet cell membrane antigens activate diabetogenic CD4+ T‑cells in the BB/Wor rat. Diabetes. 1999·May; 48(5):975‑82. <·PMID:·10331400·> | Nohara O, Kulka M, Déry RE, Wills FL, Hirji NS, Gilchrist M, Befus AD: Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide. J·Immunol. 2001·Nov·15; 167(10):5935‑9. <·PMID:·11698471·> | Mabarrack NH, Turner NL, Mayrhofer G: Recent thymic origin, differentiation, and turnover of regulatory T cells. J·Leukoc·Biol. 2008·Nov; 84(5):1287‑97. <·PMID:·18682578·>

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