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CyFlow™ CD3 PerCP

CyFlow™ CD3 PerCP
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Laser: Blue, Green
Source Species: Mouse
Field of Interest: Immunophenotyping
Antigen: CD3
Alternative Name: Leu4, T3
Clone: MEM-57
Isotype: IgG2a
Format/Fluorochrome: PerCP
Excitation Maximum: 482 to 490 nm
Emission Maximum: 678 nm
Order number: BA290102
HLDA Workshop HLDA IV—WS Code T 96 Quantity 100 tests Volume 1.0 mL Immunogen... more
Product information "CyFlow™ CD3 PerCP"
HLDA WorkshopHLDA IV—WS Code T 96
Quantity100 tests
Volume1.0 mL
ImmunogenHuman thymocytes and T lymphocytes
Background InformationCD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Technical Data Sheet

Certificate Of Analysis

ECDoC

Instruction for Use

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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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